Publication Details
Abstract
A simple, accurate, and Ultrasensitive Spectrophotometrric determination of cefotaxime (CEF) in pure form and in pharmaceutical formulations was analyzed and developed. The proposed method is based on the conjugation reaction between denaturing cefotaxime and thiosimcarbazone in a basic medium, resulting in the formation of a stable, water-soluble, bright red azo dye exhibting maximum absorbance wavelength 514 nm. The method demonstrated compliance with Beer's law over the concentration range of (5-65 μg/ml)µg/ml, achieving a limit of detection (LOD) of 0.214 µg/ml, with an (LOQ) of 0.677 µg/ml. a Sandell sensitivity of 0.084 µg/cm², and a molar absorbance of 5.681x103 L·mol⁻¹·cm⁻¹. The stoichiometric ratio of the drug substance to the reagent in the resulting stain was determined using both the Job method and the molar ratio, and was found to be 1 : 1 Cefotaxime levels in pharmaceutical formulations and pure samples were successfully determined using the suggested method.demonstrating high analytical efficiency and reliability.