Publication Details
Abstract
In recent years, the consumption of caffeine has significantly increased worldwide. However, the potential negative effects of caffeine on the reproductive system have raised concerns among researchers and health professionals. The present study aims to investigate the effects of caffeine on the histology of the liver and testes of male rabbits and caffeine relation to reproductive hormones. Twenty-one adult male rabbits Florida White were divided into three groups. The Control Group was given 2ml of normal saline, while the second (G1) was given 2ml caffeine orally at a dosage of 2 mg/ml, the third group (G2) was given 2ml caffeine orally at a dosage of 6 mg/ml, for month. At the end of the experiment, liver and testes were obtained and stained with H & E stains. Blood samples were also collected to study; thyroid stimulating hormone (TSH), follicular stimulating hormone (FSH), and luteinizing hormone (LH). Histological results revealed that caffeine cause disorganized germinal epithelium of seminiferous tubules and marked vacuolations with a pronuclear of sperm. Moreover, in the liver there were necrosis of hepatocytes, fatty degeneration. There was significant decrease in the concentrations of FSH, LH, and TSH levels in both (G1) and (G2) compared with the control group. In conclusion, caffeine had an adverse effect on liver and testes characterized by tissue degeneration, cellular necrosis, and structural disruption evidenced by restoring the normal state when the animals were banned from further caffeine treatment.